2-甲基萘[2,3-b]呋喃-4,9-酮(FNQ3)对肺腺癌细胞株(A549)细胞Fas受体表达和细胞凋亡的影响

Effect of FNQ3 on Fas Receptor Expression and Apoptosis in Lung Adenocarcinoma Cell A549

目的探讨2-甲基萘[2,3-b]呋喃-4,9-酮(FNQ3)对A549细胞表面Fas受体表达和细胞凋亡的调节效应。方法给A549细胞投与FNQ3,从药物的浓度依赖性及投药的时间依赖性两方面来观察药物对A549细胞表面Fas受体表达及诱导凋亡的作用。用半定量RT-PCR法检测FasmRNA,用流式细胞仪检测A549细胞表面的Fas受体表达和细胞凋亡。结果A549细胞表面Fas受体表达和A549细胞FasmRNA及细胞凋亡敏感度都随着药物浓度和投药时间的增加而增加。在研究药物浓度依赖性时,FNQ3浓度为2.5μg/ml时FasmRNA产量相对较高,为75000分子/μl;A549细胞表面Fas受体表达相对较多;细胞凋亡率达到37.26%(P<0.01)。在研究药物时间依赖性时,FNQ3(0.5μg/ml)投药时间为7天时,FasmRNA产量相对较高,为150000分子/μl;A549细胞表面Fas受体表达相对较多;细胞凋亡率30.45%(P<0.02)。结论FNQ3作为1种抗肿瘤药物能够诱导A549肿瘤细胞表面Fas受体表达,并上调由Fas受体介导的细胞凋亡。

Objective To study the effect of 2-Methylnaphtho[2,3-b]furan-4,9-dione(FNQ3) on Fas receptor expression and apoptosis in A549 cells.Methods The A549 cells were treated with different dose of FNQ3 for 24 hours.Also,the A549 cells received 0.5 g/ml FNQ3 for 1,2,3,7 days.The Fas mRNA was detected by QRT-PCR and the cell surface Fas receptor expression and apoptosis were analyzed using flow cytometry.Results FNQ3 increased Fas mRNA product,the expression of Fas receptor and apoptosis in a dose- and time-dependent manner.With the treatment of highest dose of FNQ(32.5 g/ml),the Fas mRNA Product in A549 cells was 75 000 molecules/μl,the expression of Fas receptor increased and the apoptosis rate reached 37.26%(P<0.01).After the treatment of 0.5 g/ml FNQ3 for 7 days,the Fas mRNA(150 000 molecules/μl),cell surface Fas receptor expression,and apoptosis rate(30.45%) reached the peak(P<0.02).Conclusion FNQ3 can increase the cell surface Fas receptor expression and up-regulate the sensitivity of Fas-mediated apoptosis in a time- and dose-dependent manner.FNQ3 can be used as anti-tumor medicine.