神经母细胞瘤细胞生物学检测和荷瘤鼠模型的建立

The establishment of neuroblastoma cell lineand experimental study for mice model

目的用新鲜肿瘤组织,建立原发瘤的神经母细胞瘤体外细胞系和不同浓度的细胞悬液,制作荷瘤鼠模型,并建立起鼠神经母细胞瘤体外细胞系。方法取新鲜肿瘤标本,用酶消化法,建立体外细胞系和细胞悬液,进行生物学检测,接种于裸鼠(n=14)和SCID小鼠(n=3)后肢前下方的皮下及腹膜后。结果人神经母细胞瘤原发瘤体外细胞系建立成功。裸鼠(5/11)和SCID小鼠(n=2/3)荷瘤成功。结论建立神经母细胞瘤细胞系和动物模型对于研究其转基因诱导分化、神经母细胞瘤的分子生物学特征及其肿瘤自消机制打下了良好的基础。

Objectives To establish the in vitro cell line and cell suspension with fresh tumor tissue and to get the bear-tumor nude mice model with the cell suspension established by ourselves.Method The fresh tumor tissue was taken from the patient,the enzyme digestion method was used to establish the in vitro cell line.The single cell suspension wsa inoculated by the mode of hypodermic injection and retroperitoneal injection for 14 SPF nude mice and 3 SCID mice.Results The in vitro cell line of primary tumor was established successfully.Now the 52th generation cell line,the bear-cancer nude mice models (5/14)and the SCID mice models(2/3)were obtained.There were two nude mice with metabasis all over the body,thrce mice with tumor at inoculated site.Conclusion The experiments can supply a very important basis for the further research to the mechanism of disappearing spontaneously and the mechanism of inducing differentiation with gene and medicine.