乙型肝炎患者树突状细胞内HBV DNA存在状况的研究

Study Whether Hepatitis B Virus Deoxyribonucleic Acid (HBV DNA)Localized in Dendritic Cells (DCs) in Patients with Chronic Hepatitis B(CHB)

目的:研究慢性乙型肝炎患者外周血培养的树突状细胞(DC)内乙型肝炎病毒DNA(HBVDNA)存在状况,分析HBVDNA与DC功能下降的内在联系。方法:从慢性乙型肝炎患者外周血中分离单个核细胞,在含rhGM CSF,rhIL4的培养基中诱导培养为DC,用流式细胞仪检测乙肝患者DC的表型变化,用流式细胞仪分选出树突状细胞,应用PCR技术检测DC内HBVDNA存在状况。结果:①表达于慢性乙型肝炎患者DC表面的共刺激分子(B7-1,B7-2,CD1α)及MHCⅡ类分子(HLA DR)的水平明显降低。②树突状细胞内HBVDNA检测结果阳性。结论:乙型肝炎患者树突状细胞内存在HBVDNA感染,慢性乙型肝炎患者树突状细胞与正常人相比,表型表达降低。

Objective:To study whether hepatitis B virus deoxyribonucleic acid(HBV DNA)localized in dendritic cells(DCs)in patients with chronic hepatitis B (CHB)and to analyze the relationship between HBV DNA and DCs functional impairment. Methods: Monocyte was isolated from peripheral blood of patients with chronic hepatitis B infection and healthy controls, further cultured into DCs in medium containing GM-CSF and IL-4. T phenotypes of DCs were checked by flow cytometry(FCM). HBV DNA in DCs was checked by polymerase chain reaction(PCR). Results: DCs in patients with chronic hepatitis B had lower expression costimulatory molecules and surface molecules CD1α,HLA-DR) than that in normal controls,and especiually CD1α exhibited more significant decrease than that in healthy controls.PCR revealed that DCs in patients with chronic hepatitis B localized HBV DNA.Conclusion:Infection of HBV DNA localizes in DCs in patients with CHB. The expression of phenotypes on DCs in patients with CHB decreases than that in healthy controls.