摘要
目的:探讨乙型肝炎病毒(HBV)基因组前C区变异和基本核心区启动子(basiccorepromoter.BCP)变异的临床意义以及基因芯片在慢性乙型肝炎(慢乙肝)临床检测中的应用。方法:用基因芯片法检测HBVDNA阳性慢乙肝患者的血清标本,根据HBV血清标志物的实验结果将受试者分为HBeAg(+)组和HBeAg(-)组,比较分析两组患者的临床资料,并根据变异在不同类型慢乙肝患者中的检出状况,以评定HBV前C区和BCP区变异的临床意义。结果:81例血清HBVDNA阳性患者中检出前C区变异51例,突变检出率为63.0%,检出BCP区变异60例,突变检出率为74.1%,其中HBeAg(-)者变异的检出率均明显高于HBeAg(+)者,突变株的检出以慢性重型肝炎最高,其次是慢乙肝重度、中度患者,慢乙肝轻度患者最低,表明HBV前C区突变与病情轻重及e系统血清标志有关,而与性别、年龄无关。结论:基因芯片定量检测HBV基因突变高效可靠,对于判断慢乙肝患者病情轻重、预后好坏和指导用药,具有一定临床参考价值。
Objective:To approach the clinical significance of Hepatistis B virus(HBV) Pre-C and basic core promoter(BCP)mutation, and the application of gene chip in the clinical detection of chronic type B hepatitis.Methods:Using gene chip to detect the serum samples of chronic hepatitis B patients with HBV DNA positive, during the analyse on the result, divided the subjects into two groups according to the data of detecting the HBV serum symbol: the HBeAg(+)group and the HBeAg(-)one, then we compared and analyzed the clinical data of the two groups to evaluate the clinical significance of HBV Pre-C and BCP mutation, also according to the different mutation rate in different type B hepatitis.Results:Gene mutation on HBV Per-C was detected in 63.0%(51/81),74.1%(60/81) in BCP. The gene mutation in HBeAg(-)group is significantly higher than HBeAg(+)group. The arte of detection in mutant stock is highest in chronic serious hepatitis and lower in midrange chronic type B hepatitis.It dedicated that gene mutation in HBV Pre-C is connected with the pathogeneitc condition and serous signal of e system and has nothing to do with sex and age.Conclusion:It is high efficient and reliability to detect the gene mutation in HBV with gene chip, and it is useful to estimate the pathogenetic condition and good for prognosisand medication.
出处
《中西医结合肝病杂志》
CAS
2005年第3期164-166,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases