胆道狭窄致胆色素结石时胆汁酸成分的变化

Changes of bile acid components in PS induced by the stenosis of bile duct

研究胆汁中胆汁酸的变化与胆色素结石形成的关系。方法结扎豚鼠的胆总管造成狭窄,一周后测定胆汁中胆汁酸及其它成分并行病理研究。结果狭窄组(S组)成石率达92.3%,均为胆色素结石(PS),胆汁中总胆红素(TBr)、非结合胆红素(IBr)、钙离子(ICa)、糖蛋白(GP)均较对照组(C组)明显升高总胆汁酸(TBA)也明显增高。S组胆汁中牛磺鹅脱氧胆酸(TCDCA)明显升高,而甘氨鹅脱氧胆酸(GCDCA)明显降低,G/T值显著下降。超微病理研究表明,S组肝细胞内线粒体明显肿胀,滑面内质网显著减少。结论胆汁G/T的下降与肝脏病变均有利于PS形成。

To study the relationship between the changes of bile acid components and pigment stonel PS, formation. Method By ligating the common bile duct to develop an animal model of bile duct stenosis in guinea pigs, the concentrations of bile acids and other compositions in bile were determined and the pathological study were performed. Result The incidence of PS formation was 92. 3yj in stenosis group. All the stones were calcium bilirubinate positive. The concentrations of TBr. IBr, ICa, GP, TBA and TCDCA in bile in stcnosis group were all significantly higher than those in control group (P< 0. 0001), while GCDCA level was obviously decreased (P<0.05), showing an obvious decrease of G/T ratio (P<0.0001). Ultrastructural observations of hepatic cells indicated that mitochondria became appar- ently swelling. smooth endoplasmic reticulum decreased significantly. Conclusion The decrease of G/T ratio and changes of the hepatic cell structures may play important roles in PS formation.