休克时组织内细胞因子的表达及其与内毒素移位的关系

Expression of TNFα,IL-1β,IL-6 in tissues and their relationship with endotoxin translocation in hemor-rhagic shock

目的探讨休克时组织内肿瘤坏死因子(TNF)α、白介素1 (IL-1)β、白介素6 (IL-6)的表达及其与内毒素移位的关系。方法应用RT-PCR、 ELISA等方法,观察小鼠和大鼠休克后肝、肺、肾、肠组织内TNFα, IL-1β、IL-6 mRNA的表达TNF α的释放和内毒素水平的变化。结果失血性休克和复苏后,各重要器官内TNFα、IL-1β、IL-6等的基因相继表达增加,以TNF α表达最早;各组织内TNFα水平也均有不同程度升高,于复苏后3小时,三种组织内TNF α水平仍显著升高;肝、肺、肾组织中内毒素水平先后明显增加,与休克早期组织内TNFα水平呈明显的相关关系。结论失血性休克及复苏后重要器官内细胞因子可相继表达、释放增多,可能与休克后内毒素的组织移位有关。

Objective To study the expression of TNF a, IL-1 P,11--6 mRNA and release of TNF a in the liver, lungs, kindneys and intestine, and their relationship with endotoxin translocation after hemorrhagic shock in mice, and rats. Methods Reverse-transcription-polymerase chain reaction and ELISA were used. Re- sult The expression of TNF α, IL-1β, IL-6 mRNA in the vital organs could be successively increased af- ter hemorrhagic shock and subsequent resuscitation, among which TNF a expression was shown to be ear- liest. TNF a levels in tissues were all elevated but in different degree after shock and resuscitation, which were shown to be still significantly higher 3 hours after resuscitation. Endotoxin levels in the liver, lungs and kidneys were markedly increased after shock and resuscitation, which were significantly correlated with TNF a levels in tissues at the early stage of shock. Conclusion The expression and release of cy- tokines in the vital organs could be successively increased after hemorrhagic shock and resuscitation, which might be related to endotoxin translocation into tissues after shock.