一氧化氮供体对犬冠状动脉阻塞后血浆 一氧化氮浓度及心肌梗塞范围的影响

Effect of nitric oxide donors on the level of plasme nitric oxide and the area of infarction in a canine coronary artery occlusion model

目的探讨血浆一氧化氮(NO)对缺血后心肌的保护作用及NO供体的治疗意义。方法将20条犬制成急性心肌梗塞模型后分成两组,治疗组给予NO供体硝酸甘油,对照组用5%葡萄糖注射液静脉滴注。两组在冠状动脉结扎前后各时间点采静脉血测血浆NO浓度,两天后处死动物,取出心脏用TTC染色法测量梗塞面积。结果两组犬冠状动脉结扎后血浆NO浓度明显低于结扎前,但治疗组高于对照组并且心肌梗塞面积明显缩小。结论犬冠状动脉阻塞后血浆NO浓度降低,其程度与心肌梗塞面积大小有密切关系。应用NO供体后,可能提高血浆NO浓度并使梗塞面积减小。

Objectives To detact the protactive action of nitric oxide (NO) donors on ischemic myocardium. Methods Twenty canines with lasting ligation of left anterior descending coronary artery (LAD) werc divided into two groups. nitroglycerin in the group I (n=10) and 5 0 o glucose solution in the group ll (n=10) were administered intravenously after I_AD occlusion. The level of the plasme NO was measured in some time points before and after LAD were ligated in two group canines. After two day all dog hearts were taken out to measure the infarct size by triphenyltetrazolium (TTC) staining. Results After LAD were ligated the levels of the NO were lower than those before I_AD ligated in all dogs. In group II they were lower and the area of myocardial infarction was signif, cantly increase than those in group I . Con- clusions The level of plasme NO is decrease after canines coronary artery occlusion and it is related to the size of infarction. The NO donors can increase the level of the plasme NO and decrease the infarction area.