PD98059对乳腺癌细胞的抗癌效应

The Anticancer Effect of PD98059 on Breast Cancer Cells

目的研究PD98059对乳腺癌细胞株的抗肿瘤效应。方法用50μmol·L1PD98059处理乳腺癌细胞系MCF-7(ER+/PR+)和MDA-MB-435s(ER-/PR-)1h后,采用逆转录酶聚合酶链反应(RT-PCR)检测AP-1、cyclinD1的mRNA水平,采用AnnexinV/PI法检测细胞凋亡,PI染色流式细胞仪检测细胞周期。结果PD98059明显抑制两种乳腺癌细胞的AP1和cyclinD1的mRNA水平,促进两种细胞的凋亡,并阻滞两种细胞的G1/S期转换。结论PD98059对乳腺癌细胞具有明显的抗肿瘤效应,可能成为乳腺癌靶向性化疗新药。

Objective To study the anticancer effect of PD98059 on human breast cancer cell lines. Methods Human breast cancer cells MCF-7(ER+/PR+) and MDA-MB-435s(ER-/PR-) were treated with 50 μmol·L -1 PD98059 for 1 h, and the mRNA levels of AP-1and cyclin D 1, apoptosis and the cell cycle of the cancer cells were then assayed with RT-PCR, AnnexinV/PI method and color flow cytometry,respectively. Results PD98059 was shown to exert striking inhibitory effect on the mRNA levels of AP-1 and cyclin D 1. It also promoted apoptosis and blocked the G 1/S cycle conversion of both kinds of cancer cells. Conclusion PD98059 was shown to have dramatic antineoplastic effects on human breast cancer cells and may become a potential chemotherapeutic drug for breast cancer.