甲心安煎剂对甲状腺素性心脏肥大大鼠心肌胶原含量的影响

The Effect of Jiaxinan Decoction on the Content of Myocardial Collagen in a Rat Model of Thyroxin-induced Myocardial Hypertrophy

目的探讨甲心安煎剂治疗甲状腺素性心脏肥大的机制。方法将清洁级SD大鼠40只分为4组,即正常组、模型组、甲心安高剂量和低剂量治疗组各10只。正常组与模型组每日灌服纯化水2mL,高剂量组每日灌服甲心安原液(含生药4g·mL1),低剂量组灌服甲心安稀释液(将原液加纯化水稀释至含生药2g·mL1),连续给药10d。实验第11天取标本检测指标。结果模型组左心室重量及与体重之比均高于正常组(P<0.01),心肌胶原含量及Ⅰ/Ⅲ胶原比例均较正常组高(P<0.01)。与之相反,经过甲心安煎剂干预后,治疗组无论低剂量或高剂量,其上述指标均较模型组低(P<0.01)。结论甲心安煎剂能改善甲状腺素引起的心肌肥厚,降低重构心肌中的胶原含量,并调整优化心肌中Ⅰ/Ⅲ胶原比例。

Objective To probe into the mechanisms underlying the effect of jiaxinan in the treatment of myocardial hypertrophy induced by thyroxin in the rat. Methods 40 adult SD rats were randomly divided into 4 equal groups: group A, normal control; group B, model; group C, model+high dose jiaxinan ; group D ,model+low dose jiaxinan.A model of myocardial hypertrophy for each rat of group B,C and D was set up by a daily hypodermic injection of 1 mg·kg -1 of L-thyroxin for 10 consecutive days. Beginning from the 6 th day of the experiment, rats of group A and B were given each 2 mL of pure water by gastrogavage, q.d.,for 11 consecutive days .Meanwhile, animals of group C and D were given each 2 mL of the stock solution of jiaxinan (containing 4 g·mL -1 of the crude drug )and 2 mL of 1∶2 diluted stock solution of jiaxinan(containing 2 g·mL -1 of the crude drug ) by gastrogavage ,q.d., respectively, for 11consecutive days. Rats of all 4 groups were then sacrificed and heart samples taken for examination . Results The average ratio of the weight of the left ventricle to the body weight in rats of group B(model)was higher than that in rats of group A (normal control)(P<0.01).The myocardial content of collagen and the Ⅰ/Ⅲ collagen ratio in rats of group B(model) were higher than those in rats of group A(P<0.01).In contrast ,these parameters in rats of group C and D that had been treated with high and low dose jiaxinan were lower than those of group B (model)(P< 0.01). Conclusion The traditional Chinese medicine jiaxinan was shown to alleviate myocardial hypertrophy induced by thyroxin in rats .It also lowered the myocardial content of collagen, modulated and optimized the Ⅰ/Ⅲ collagen ratio in the murine myocardium .