骨髓间充质干细胞治疗皮肤深Ⅱ度烧伤的实验研究

Experimental study on treatment of deep partial thickness derma burn injury using bone marrow mesenchymal stem cells

目的:观察骨髓间充质干细胞(MSCs)对皮肤深 度烧伤的疗效。方法:体外培养扩增雄性豚鼠骨髓MSCs,以2×10 6 ·m L- 1 、2×10 7·m L- 1 细胞密度,移植给皮肤深 度烧伤的雌性受体鼠创面,对创面的愈合面积进行计算,用HE、Masson染色进行组织形态学观察。结果:治疗组的皮肤创面修复较好,HE染色结果显示治疗组的真皮与表皮结构清晰,表皮各层清楚,而对照组的皮肤创面细胞各层次不清楚。Masson染色结果显示治疗组皮肤创面新生被覆上皮内,胶原纤维排列较整齐;对照组胶原增粗、排列不规则。2×10 6 ·m L- 1 、2×10 7·m L- 1 不同细胞密度的治疗侧之间比较差异无显著性。结论:骨髓MSCs可促进皮肤深 度烧伤的愈合,对皮肤深 度烧伤具有明显的疗效。

Objective To observe the curative effects of deep partial thickness derma burn injury using bone marrow mesenchymal stem cells. Methods The bone marrow stem cells were obtained from the male guinea pig and cultured in vitro. Then the mesenchymal stem cells (MSCs) with 2×10 6 cell·mL -1 and 2×10 7 cell·mL -1 were prepared, and transplanted into the wounds of the female guinea pig models with deep partial thickness derma burn injury. The wound healing area was counted and the morphological changes were observed by using HE and Masson stain. Results The wound healing in therapy groups were significantly better than that in control groups. HE stain showed that dermal and epidermal structures were clear, and cell structures of every epidermal layers were distincted and integrated in therapy groups,but cell structures of every layers in control groups were unclear. Masson stain showed that collagen was regular and new-born epidermal covered the wound in therapy groups, collagen was disorder and thicker in control groups. Bone marrow MSCs could promote the healing of wound and the effects in therapy groups were better than that in the control groups. But there was no significantly difference between MSCs with 2×10 6 cell·mL -1 and 2×10 7 cell·mL -1 in therapy groups. Conclusion The bone marrow MSCs have significant curative effects on deep partial thickness derma burn injury.