局灶性脑缺血及再灌注损伤IGF-Ⅰ表达的动态变化被引量：7

Changes of IGF-Ⅰ expression in focal cerebral ischemical reperfusion injury model of rats

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摘要目的:探讨胰岛素样生长因子- (IGF- )在局灶性脑缺血再灌注损伤中的表达及作用。方法:采用线栓法制备大鼠局灶性脑缺血模型,应用免疫组化染色、原位杂交技术检测IGF- 蛋白及m RNA表达。结果:正常组织有极低水平的IGF- 表达,脑缺血后表达主要位于半影区,随缺血及缺血再灌注时间延长,半影区表达逐渐增强,IGF- 蛋白及m RNA表达在再灌注2 4 h为16 9.6 4±6 .97、16 8.6 4±6 .2 8,4 8h为16 8.86±5 .39、170 .86±5 .0 7,并达高峰。结论:内源性IGF- 表达增强,对局灶性脑缺血再灌注损伤具有保护作用。 Objective To study the expression of insulin-like growth factor-Ⅰ (IGF-Ⅰ) in focal cerebral ischemical reperfusion injury model and its contribution. Methods The focal cerebral ischemia rat models were induced using the filament method, the expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA was dectected by the method of immunohistochemical staining and in situ hybridization technique. Results The expression of IGF-Ⅰ was very low in normal tissues, while increased mainly in penumbra after cerebral ischemia. The expressions of IGF-Ⅰ protein and IGF-Ⅰ mRNA were 169.64±6.97 and 168.64±6.28,respectively, at 24 h after reperfusion and peaked to 168.86±5.39 and 170.86±5.07 at 48 h after reperfusion. Conclusion Enhanced expression of endogenous IGF-Ⅰ has protective effects on focal cerebral ischemia reperfusion injury.

作者徐忠信何金婷莽靖胥桂华杨宏钱佳利

机构地区吉林大学中日联谊医院神经内科长春中医学院生理学教研室

出处《吉林大学学报（医学版）》 CAS CSCD 北大核心 2005年第3期401-403,共3页 Journal of Jilin University：Medicine Edition

基金吉林省科技厅资助课题 (2 0 0 30 5 36 - 5 ) 吉林大学中日联谊医院重点资助课题 (2 0 0 2 0 1- 6 )

关键词脑缺血再灌注损伤胰岛素样生长因子-Ⅰ 半影区 brain ischemia reperfusion injury insulin-like growth factor-Ⅰ penumbra

分类号 R-332 [医药卫生] R742 [医药卫生—神经病学与精神病学]

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