TGF-β1和TβRⅡ在胰腺癌组织中的表达及临床意义

The expression of TGF-β1 and TβRII in human pancreatic ductal adenocarcinoma and its significance

目的研究转化生长因子β1(TGF-β1)及其Ⅱ型受体(TβRⅡ)在人胰腺癌组织中的表达,探讨其与胰腺癌进展、转移的关系。方法应用SABC免疫组化方法检测TGF-β1、TβRⅡ在10例正常胰腺、13例慢性胰腺炎和36例胰腺癌中的表达。结果TGF-β1、TβRⅡ阳性率在正常胰腺均为10.0%,慢性胰腺炎中分别为7.7%、15.4%,胰腺癌中分别为44.4%、47.2%,胰腺癌中的阳性率明显高于前两组(P<0.05);TGF-β1、TβRⅡ的表达与胰腺癌患者的年龄、性别、肿瘤的大小、位置、组织学分级无关(P>0.05),与临床分期相关(P<0.01);TGF-β1、TβRⅡ在胰腺癌中共同阳性率为36.1%,其共同表达与胰腺癌的组织学分级和临床分期有关(P<0.01)。结论单独检测TGF-β1、TβRⅡ的表达对判断胰腺癌的进展、转移趋势有参考价值;两者共同表达对判断胰腺癌的恶性程度及进展、转移趋势有参考价值。

Objective To explore the expression of TGF-β1 (transforming growth factor-β1) and TβRII(transforming growth factor-βreceptorII) in pancreatic cancer. Methods TGF-β1 and TβRII were examined immunohistochemically using strepto-advidinbiotin complex (SABC) method in pancreatic specimens of 36 patients with pancreatic ductal adenocarcinoma, 13 patients with chronic pancreatitis and in 10 specimens of normal pancreas. Results The positive rate of TGF-β1 was 44.4%,7.7% and 10.0%; the positive rate of TβRⅡ was 47.2%,15.4% and 10.0%,in pancreatic cancer,chronic pancreatitis and normal pancreas respectively. The coexpression rate of TGF-β1 and TβRⅡ in pancreatic cancer (1336, 36.1%) was increased significantly compared with chronic pancreatitis and normal pancreas (P<0.05). TGF-β1 and TβRⅡ expression was not related to the age,sex of patients,and site,size and histopathological grade of tumors (P>0.05), but related to clinical stage of tumor (P<0.01). The coexpression in pancreatic cancer was related to the histopathological grade and clinical stage of tumors (P<0.01). Conclusion The detection of TGF-β1 and TβRⅡ expression in the human pancreatic cancer will be useful in assessment of its clinical status.