幽门螺杆菌感染与不同胃粘膜病变中基质金属蛋白酶-9表达的关系

Relationship between MMP-9 Expression and H.pylori Infection in different Gastric Mucosa Lesions

目的 探讨基质金属蛋白酶9(MMP 9)在胃癌及不同胃粘膜病变发生过程中的表达,及其与幽门螺杆菌的相互作用。方法 选择2 0 9例病例,包括慢性胃炎2 0例、肠上皮化生18例、轻度不典型增生4 1例、中重度不典型增生76例及胃癌5 4例;HP检测采用Giemsa染色、快速尿素酶试验或14 C尿素呼气试验;SP法检测MMP 9的表达。结果 慢性胃炎粘膜组未见MMP 9表达,肠上皮化生粘膜组MMP 9阳性率为4 4 .4 % ,轻度不典型增生粘膜组MMP 9阳性率为4 8.9% ,中、重度不典型增生粘膜组MMP 9阳性率为71.1% ,胃癌组MMP 9阳性率为83.3% ,明显高于慢性胃炎粘膜组(P <0 .0 1) ,高于不典型增生粘膜组(P <0 .0 1)。MMP 9在不典型增生及胃癌的HP阳性组中的表达率明显高于HP阴性组(P <0 .0 5 )。结论 MMP 9在胃癌中有较高的阳性率,与HP感染密切相关,可作为诊断胃癌的一个参考指标。

Objective To evaluate the expression of matrix metalloproteinase-9(MMP-9) in gastric carcinoma and different gastric mucosa lesions,and the relationship between MMP-9 and H.pylori.Methods A total of 209 cases were available for the study including 20 cases of chronic gastritis,18 cases of intestinal metaplasia,41 cases of mild dysplasia,76 cases of modest or severe dysplasia and 54 cases of gastric carcmoma;H.pylori was determined with the rapid urease test,Giemsa staining method or ^(14)C urease breath test;And streptavidin-peroxidase (SP) immunohistochemical method was used to detect the expression of H.pylori.Result The positive rate of MMP-9 expression in intestinal metaplasia,mild dysplasia,modest or severe dysplasia and in gastric carcinoma were 44.4%,48.9%,71.1%,83.3%,respectively significantly higher than that in chronic gastritis (P<0.01) and that in dysplasia (P<0.01).The positive rate of MMP-9 in dyplasia and gastric carcinoma with HP infection were significantly higher than those without infection.Conclusion The positive rate of MMP-9 was high in gastric cancer and may serve as a marker for diagnosing gastric cancer.There is obvious correlationship between MMP-9 expression and HP infection.