骨肉瘤中PCNA和AgNOR的表达与其预后关系的研究

Study of the expression of PCNA and AgNOR in osteosarcoma and their relationship to the prognosis of this disease

目的:探讨骨肉瘤中增殖细胞核抗原(PCNA)和银染核仁组成区(AgNOR)的表达及与其预后的关系。方法:选1998年1月～1999年1月骨肉瘤患者术后石蜡标本41例,分别采用S-P免疫组化法及Ploton一步银染法行PCNA及AgNOR染色。结果:PCNA指数与AgNOR计数呈正相关(P<0.05);恶性度高者的PCNA指数与AgNOR计数高于恶性度低者;<3年术后生存期者高于>3术后生存期者。结论:PCNA及AgNOR在骨肉瘤中表达具有明显的相关性,二者可作为一种新的对骨肉瘤预后估计的指标。

Objective:To investigate the expression of proliferating cell nuclear antigen(PCNA)and Ag-nuclear organizer region(AgNOR)in osteosarcoma and their relationship to the prognosis of this disease.Methods:41cases of osteosarcoma from January1998to January1999in our hospital were stained histochemistrily by S-P immunohistochemistry of PCNA and ploton AgNOR methods respectively.Results:The index of PCNAwas positively correlated with the count of AgNOR(P<0.05).The index of PCNA and the count of AgNORwere higher in the osteosarcoma patients with high malignancy than in osteosarcoma patients with lowmalignancy,whichwere higher in the osteosarcoma patients<3-year survival period than in the osteosarcoma patients>3-year survival period.Conclusion:PCNA and AgNOR can be used as an index in predication the prognosis of osteosarcoma.