吴茱萸次碱促进脑组织降钙素基因相关肽释放减轻脑缺血-再灌注损伤

Rutaecarpine Ameliorates Cerebral Ischemia-reperfusion Injury by Stimulating Calcitonin Gene-related Peptide Release in the Rat Brain

目的观察吴茱萸次碱对大鼠脑缺血-再灌注损伤及脑组织降钙素基因相关肽的影响。方法大鼠实验前30min静脉注射吴茱萸次碱(50、100、300μg·kg-1),然后用线栓法制作大鼠局灶性脑缺血-再灌注损伤模型,缺血2h,再灌注24h。于再灌注后6、12、24h进行神经功能缺陷评分;采用TTC染色法测定脑梗死体积;放射免疫法检测脑组织匀浆中降钙素基因相关肽(CGRP)含量。结果吴茱萸次碱呈剂量依赖性减少脑梗死体积并改善功能预后,与溶媒组比较有显著性差异;各吴茱萸次碱治疗组脑梗死后CGRP含量和溶媒组比较显著增高。结论吴茱萸次碱对缺血性脑损伤有明显保护作用,其机制可能与促进脑组织CGRP的释放有关。

Objective To investigate the effects of rutaecarpine on the cerebral injury and calcitonin gene-related peptide (CGRP) level during focal cerebral ischemia-reperfusion injury in rats. Methods Rats were intravenously given three doses of rutaecarpine (50, 100 and 300μg/kg) or vehicle 30 minutes before experiment. Then the rats subjected middle cerebral artery occlusion (MCAO) for 2 hours, and neurological deficits scores were performed at 6, 12 and 24 hours after reperfusion. After the last test the animals were sacrificed, the infarct volumes were determined by TTC staining, and CGRP levels were measured by radioimmuoassay. Results Rutaecarpine significantly reduced infarct volume, and improved cerebral function in dose-dependent manner compared with vehicle. Rutaecarpine significantly inecreased brain CGRP levels after reperfusion as well. Conclusion Rutaecarpine has marked protective effects on ischemic brain damage in rats possibly by increasing CGRP release in the brain.