山莨菪碱对海水淹溺性肺水肿的治疗作用

Therapeutic Effect of Anisodamine on Experimental Pulmonary Edema After Sea-water Drowning

目的探讨山莨菪碱对海水淹溺性肺水肿(PE-SWD)治疗作用。方法复制大鼠PE-SW模型后,将大鼠随机分为手术对照组(C组)、PE-SW模型组(M组)和山莨菪碱治疗组(T组)。采用全自动动脉血气分析仪测定各组大鼠动脉血气,使用酶联免疫吸附试验法(ELISA)测定个组大鼠血浆、支气管肺泡灌洗液(BALF)和肺组织中TNF-α含量,同时测定个组大鼠肺组织湿/干重变化。结果海水灌注后2h开始,T组大鼠PaO2、SO2、pH显著高于M组。肺组织湿/干重比值则低于M组;M和T组TNF-α在血浆、BALF和肺组织的浓度与对照组相比在淹溺后显著升高,1h到达峰值,后逐渐下降。海水灌注后,T组大鼠TNF-α均明显低于M组大鼠。结论山莨菪碱对海水淹溺性肺水肿有治疗作用,它的作用可能是通过对TNF-α的影响而产生的。

Objective To study the therapeutic effect of anisodamine on pulmonary edema after sea-water drowning(PE-SWD). Methods Rat models of PE-SWD were set up by infusing sea-water into rats lungs,and then were randomly divided into PE-SWD model group(M group) and therapeutic group with anisodamine(T group). Blood-gas indexes of arterial blood were measured at 0.5, 1, 2, 4 and 6 hours after infusion. TNF-α levels in the plasma,bronchoalveolar lavage fluid (BALF) and lung tissues were detected by ELISA. At the same time,the dry/wet weight of lung was also measured. Results The levels of blood PaO_2,SO_2 and pH in T group were obviously higher than those in M group 2h after infusion. The dry/wet weight of lung in T group was lower than that in M group. The concentration of TNF-α in plasma, BALF and lung tissues in the both M and T groups significantly higher than that in control group, and reached maximum 1h after infusion. After infusion,the TNF-α contentration in all tested specimens in T group was obviously lower than that in M group. Conclusion There was the therapeutic effect of anisodamine on the pulmonary edema after sea-water drowning possibly by decreasing TNF-α level.