人成骨细胞胰岛素受体底物的表达

The Expression of Insulin Receptor Substrate in Human Osteoblast

目的观察人成骨肉瘤MG-63细胞和正常人成骨细胞(humanosteoblast-likecellsHOB)胰岛素受体底物(insulinreceptorsubstrateIRS)的表达。方法半定量RT-PCR检测IRSmRNA表达,免疫共沉淀检测IRS蛋白表达。结果人成骨肉瘤MG-63细胞和正常人成骨细胞均有IRS-1、-2、-3、-4mRNA和蛋白质的表达,以IRS-1的表达丰度最高,IRS-4表达丰度最低。其中IRSmRNA和蛋白质在MG-63细胞中的表达丰度较在正常人成骨细胞中的表达丰度稍高。结论人成骨肉瘤MG-63细胞和正常人成骨细胞均可表达IRSmRNA和蛋白质。且IRS不同成员在同一细胞中的表达丰度不同。

Objective To determine the expression of insulin receptor substract(IRS) family in human osteosarcoma cell line MG-63 and cultured normal human osteoblast-like cells (HOB). Methods The mRNA and protein expression of IRS family was measured using semi-quantitative RT-PCR and western blot analysis, respectively. Results MG-63 cells and HOB had the mRNA and protein expressions of IRS-1,-2,-3, and -4. Among IRS family, the expressions levels of IRS-1 mRNA and protein were the highest, and those of the IRS-4 mRNA and protein were the lowest in MG-63 cell line and HOB. Conclusion MG-63 and HOB can express the mRNA and protein of IRS family members, and the expressional levels of IRS family members were different.