hTRT和bax在甲状腺乳头状癌中的表达

Expression of hTRT and Bax in Papillary Thyroid Carcinoma

目的研究不同类型甲状腺乳头状癌(PTC)中的人端粒酶逆转录酶(hTRT)mRNA和bax蛋白质的表达及意义。方法hTRTmRNA的检测采用原位杂交方法;bax蛋白质的检测采用免疫组化方法。结果hTRTmRNA在PTC、微小癌、腺内型、腺外型及癌旁组织中阳性表达率分别为86.21%、68.97%、93.33%、96.43%和41.79%;癌组织中hTRTmRNA的阳性表达显著高于癌旁组织(P<0.001);而各癌组间hTRTmRNA的阳性表达随PTC浸润程度增加而增强(P<0.05);bax在PTC、微小癌、腺内型、腺外型及癌旁组织中阳性表达率分别为72.41%、62.07%、76.67%、78.57%和55.22%,癌组织中bax的阳性表达显著高于癌旁组织(P<0.005);而各癌组间bax的阳性表达随PTC浸润程度增加而增强(P<0.05);在75例hTRTmRNA阳性病例中bax有58例阳性,hTRTmRNA和bax表达相关(P<0.05)。结论hTRTmRNA与bax蛋白质的表达均与PTC的浸润程度有关,hTRTmRNA与bax蛋白质的表达具有一致性;检测hTRTmRNA对PTC的诊断及其预后评估具有辅助价值。

Objective To study the expressions of human telomerase reverse transcriptase (hTRT) mRNA and Bax protein in different types of papillary thyroid carcinoma (PTC). Methods hTRT mRNA was detected using in situ hybridization, and Bax protein was detected by immunohistochemistry. Results The positive rates of hTRT were 86.21%,68.97%, 93.33%, 96.43% and 41.79% in PTC, papillary microcarcinoma, intra-thyroid carcinoma, extra-thyroid carcinoma and adjacent tumor tissues, respectively. The positive rate of hTRT expression in the cancer tissues was significantly higher than that in the adjacent tumor tissues (P<0.001). The hTRT expression was positively related with the invasive degree of primary cancer (P<0.05). The positive rates of Bax protein were 72.41%, 62.07%, 76.67%, 78.57% and 55.22% in PTC, papillary microcarcinoma, intra-thyroid carcinoma, extra-thyroid carcinoma and adjacent tumor tissues, respectively. The Bax protein expression in the cancer tissues was significantly higher than that in the adjacent tumor tissues (P<0.005), and positively correlated with the invasive potential of primary cancer (P<0.05). Among 75 hTRT positive cancers, 58 cases expressed Bax protein, and there was a significant relation between hTRT and Bax expressions (P<0.05). Conclusion The expression of hTRT and Bax was significantly related with the invasion potentials of PTC. hTRT mRNA expression was associated with Bax expression in PTC. The detection of hTRT mRNA may be helpful for diagnosis and prognosis evaluation of PTC.