联合检测血清Leptin、TNF-α、SOD对NASH的临床意义

Combined Assay of Serum Leptin、TNF-α、SOD In Diagnosis of Nonalcoholic Steatohepatitis

目的:探讨血清瘦素(Leptin),肿瘤坏死因子α(TNF-α),超氧化物岐化酶(SOD)浓度变化对非酒精性脂肪性肝炎(NASH)诊断及预后判断的临床应用价值。方法:采用放射免疫法检测LeptinTNF-α,化学法检测SOD。结果:(1)NASH组瘦素浓度(13.320±5.323ng/ml)显著高于NASFL组(8.068±2.320ng/ml)(P<0.05)和NC组(3.533±1.686ng/ml)(P<0.05),NASFL组瘦素浓度显著高于NC组(P<0.05)。(2)NASH组TNF-α浓度(0.322±0.165ng/ml)显著高于NC组(0.254±0.093ng/ml)(P<0.05),NASH组与NASFL组TNF-α浓度(0.271±0.098ng/ml)比较差异无显著性(P>0.05),NASFL组与NC组TNF-α浓度比较差异无显著性(P>0.05)。(3)NC组SOD浓度(112.200±7.978U/ml)显著高于NASH组(72.540±1.325U/ml)(P<0.05),NAS FL组SOD浓度(109.900±8.903U/ml)显著高于NASH组(P<0.05),NASFL组与NC组SOD浓度比较差异无显著性(P>0.05)。结论:Leptin≥10.35ng/ml,伴有TNF-α明显升高。

Objective:To evaluate combined assay of serum Leptin,TNF-α,SOD in diagnosis and prognosis of nonalcoholic steatohepatitis(NASH).Method:Chemical and radio immunoassay were used to determine the level changes of Leptin,TNF-α and SOD.Results:(1) Compared with the normal control group(3.533±1.686ng/ml),serum Leptin levels were significantly elevated(P<0.05)in the nonalcoholic simple fatty liver (NASFL) group(8.068±2.320ng/ml)and the nonalcoholic steatohepatitis(NASH)group(13.320±5.323ng/ml).Level of Leptin was much higher in the NASH group than in the NASFL group(P<0.05).(2)Serum TNF-α was much higher in the NASH group(0.322±0.165ng/ml)than in the normal control group(0.254±0.093ng/ml P<0.05)and the NASFL group(0.271±0.098ng/ml).There were no obviously differences in serum TNF-α between the normal control group and the NASFL group(P>0.05).(3) Compared with the normal control group(112.200±7.978U/ml),level of SOD was significantly dropped(P<0.05)in the NASH group(72.540±1.325U/ml).There were no obviously differences in serum SOD between the normal control group and the NASFL group(109.900±8.903U/ml,P>0.05).Conclusion:Leptin 10.35ng/ml with higher level TNF-αand lower SOD was useful marker for diagnosis of NASH.