摘要
目的:观察碱性成纤维细胞生长因子缓释微球对兔骨髓基质干细胞(BMSC)增殖的长期效应。方法:在细胞培养技术下,不加bFGF或将游离bFGF或bFGF-聚乳酸-羟基乙酸共聚物(PLGA)微球加入第二代兔骨髓间充质干细胞培养液中,用细胞计数法、四甲基偶氮唑盐微量反应比色法(MTT法)、流式细胞仪观察细胞在第1,2,4,7及10天增殖的状况,并进行统计学分析。结果:培养1d后,三组细胞计数、吸光度(OD)值差异均无显著性意义;2d时,bFGF组的MSCs细胞数高于其余二组,差异有显著性意义(q检验,P<0.05);4d时,各组MSCs细胞依次为:PLGA微球组>bFGF组>空白对照组,各组间差异均有显著性意义(q检验,P<0.05);培养7d、10d时,微球组与上述两组仍有显著性差异。流式细胞仪检测显示,培养2d时,bFGF组的G2/M+S数百分数最高,4d后PLGA微球组G2/M+S数百分数最高。结论:微球通过较长时间持续释放活性bFGF,能长期显著地促进兔骨髓间充质干细胞的增殖。
Objective:To evaluate the long-term effects of controlled release bFGF microspheres on proliferation of rabbit bone mesenchymal stem cells.Methods:The secondary cultured rabbit bone mesenchymal stem cells were divided into three groups according to the different ingredients being added to the L-DMEM culture medium,ie,nothing,bFGF group,controlled release bFGF microspheres group.The proliferation of the cultured rabbit bone mesenchymal stem cells was measured withcell counting method.MTT method and flow cytometry.Results:The in vitro cellular study showed no significant difference in the cell number and cell viability of three groups one day after plate culture.The cell number and cell viability in the bFGF group were more than those in other two groups tow days after plate culture.The cell number and cell viability those in the controlled release bFGF microspheres group wre more than those in the bFGF group and blank group four days after plate culture with significant difference.There wete still significant difference between the controlled release bFGF microspheres group and other two groups.The flow cytometrical examination showed that the G 2/M+ S percentage in the bFGF group reached the highest two days after plate culture and the G 2/M+S percentage in the bFGF-PLGA-Ms group went the highest four days after plate culture.Conclusion:bFGF-PLGA-Microphere can promote the proliferation of the mesenchymal stem cells through a long period of controlled release of BfGF.
出处
《华西医学》
CAS
2005年第2期301-303,共3页
West China Medical Journal
