中国大陆科学钻探(CCSD)微生物研究——地下3900米一株细菌的分离与鉴定

Microbiology research of the Chinese Continental Scientific Drilling:Isolation and identification of one bacterial strain from a 3900-meter-deep subsurface rocks.

在中国大陆科学钻探科钻一井的3911.28米处利用厌氧培养基分离到一株细菌CCSDFL3900,该菌的最适生长温度为65℃,兼性厌氧,革兰氏染色为阴性,端生芽孢,不运动,电镜观察无鞭毛。利用细菌16SrDNA通用引物对其16SrRNA进行PCR扩增,16SrRNA序列及其进化树分析结果显示,该菌为芽孢杆菌或地杆菌。生化试验检测显示,该菌株能够利用多种糖类发酵产酸,最终鉴定为热坚芽孢杆菌。

A bacterial strain named CCSDFL3900 was isolated from a 3911.28-meter-deep subsurface rock from the main borehole of Chinese Continent Scientific Drilling. It was Gram negative with no flagellalt has spores in the end, is immobile, facultative anaerobic, and grows optimally at 65 centigrade. Its 16S ribosome DNA was extracted and amplified with the universe primer of bacterial 16S ribosome DNA. Phylogenetic analysis results revealed that it belongs to the genus bacillus or geobacillus. Fermentation test further identified as it a strain of Bacillus caldotenax.