乌龟胃肠胰系统内分泌细胞的免疫组织化学研究

IMMUNOHISTOCHEMICAL STUDY ON ENDOCRINE CELLS IN THE GASTRO-ENTERO-PANCREATIC SYSTEM OF CHINEMYS REEVESII

应用过氧化物酶标记的链霉卵白素(Streptavidin peroxidase,简称S-P法)免疫组织化学技术,使用六种特异性胃肠激素抗血清对乌龟胃肠胰系统内分泌细胞的种类、定位、分布密度及形态进行了研究。在乌龟胰腺中检测出5-羟色胺、生长抑素、胰高糖素和胰多肽等4种内分泌细胞,生长抑素、胰高糖素细胞多成簇大量分布于胰岛中;5-羟色胺、胰多肽细胞多散在少量分布于胰腺腺泡之间。在乌龟消化道中共检测出5-羟色胺、生长抑素、胃泌素、胰高糖素和P物质等5种内分泌细胞:5-羟色胺细胞在消化道各段均有分布,以十二指肠处分布密度最高(30.7±4.2),空肠其次,回肠、直肠处最低(12.0±1.0/11.2±3.0);生长抑素细胞仅分布于食道和胃中各段;胃泌素细胞分布于胃幽门部和十二指肠处;胰高糖素细胞分布于胃体至空肠段,以胃幽门部分布密度较高(11.3±1.1);P物质细胞仅布于胃幽门部;消化道各段均未检出胰多肽细胞。与其他爬行动物比较,乌龟胃肠胰系统内分泌细胞的分布既存在着一定共同点,又显示了较大的种间差异。

Identification,localization,distribution and morphology of edocrine cells in the gastro-entero- pancreatic system of Chinese three keeled pond turtleChinemys reevesii were studied in streptavidin peroxidase(S-P)immunohistochemical method with six gut hormone antisera.Four types of edocrine cells were founded in the pancreas of C.reevesii,namely:5-Hydroxytryptamine(5-HT),somatostatin (SS),glucagon (Glu),polypeptidem (PP)edocrine cells.SS and Glu cells mainly distributed collectively in the pancreas islet with large quantity.On the other hand 5-HT and PP cells scatterly located in the pancreas alveoli with small quantity.Gastrin(Gas)and Substance P(SP) cells were not detected in the pancreas of C.reevesii,Five types of edocrine cells were obsesued in the digestive tract of C.reevesii, they were 5-HT,SS,Gas,Glu and SP cells.5-HT cells distributed throughout the digestive tract,with the highest density in the duodenum(30.7±4.2),the second highest in the jejunum,and the lowest in the Ileum and rectum(12.0±1.0/11.2±3.0).SS cells mainly located in the each region of stomach,and occasionally occurred in the esophagus.Gas cells were mainly detected in the stomachus pyloricus and also have a few in the duodenum.Glu cells were presented from stomachus corpus to jejunum with the highest density in the stomachus pyloricus(11.3±1.1).Substance P cells were only found in the stomachus pyloricus. Cells showing immunoreactivity to PP were not detected in the digestive tract of C.reevesii.Comparing with other reptiles ,some common features in distribution of endocrine cells in the gastro-entero-pancreatic system of C.reevsii were found,which exist in digestive physiology,but the difference among them was also significant.