EHF疫苗接种者IL-12、sICAM-1、CD28分子及B7-2基因检测

Expression of IL-12 and sICAM-1 in sera and CD28 on lymphocytes and detection of B7-2 gene after inoculation of EHF vaccine

目的 探讨EHF疫苗接种机体后细胞免疫应答的机制。方法 EHF双价灭活疫苗接种健康志愿者,采集接种前后静脉血分离血清和淋巴细胞。ELISA法检测免疫前后血清中IL 12、sICAM 1表达水平;IFA测定免疫前后淋巴细胞上CD2 8的表达;PCR SSP检测免疫前淋巴细胞的B7 2基因。结果 免疫后血清IL 12、sICAM 1表达水平升高,单因素方差分析有统计学差异;免疫前后淋巴细胞上CD2 8表达,χ2 检验示有显著性差异;PCR SSP方法检测免疫前淋巴细胞B7 2基因阳性5人,其中汉族3人,侗族1人和布依族1人。结论 EHF灭活疫苗接种健康志愿者后,显示机体产生了Th细胞介导的免疫应答。

Objective The purpose of this study is to investigate the mechanism of Th cells immune response after inoculation of EHF vaccine.Methods The volunteers were inoculated the EHF vaccine three times.The sera and lymphocytes of the volunteers were collected:The concentration of IL-12 and sICAM-1 in sera was measured by ELISA;The expression of CD28 on lymphocytes detected by IFA;B7-2 gene of lymphocytes before inoculation was checked by PCR-SSP.Results Compared with the levels of IL-12 and Sicam-1 in sera before and after inoculation,which is with statistical significance;Compared with the expression of CD28 after inoculation,the augment levels were of substantial significane.B7-2 gene of lymphocytes before inoculation was checked by PCR-SSP,there were 5 cases of B7-2 positive ,ie,3 of Chinese han、1 of Chinese tong and 1 of Chinese buyi.Conclusions The volunteers were succeedfully induced the Th cells immune response after inoculation of EHF vaccine.