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转录后基因沉默(PTGS)及其在作物遗传改良中的应用 被引量：25

Post-transcriptional Gene Silencing(PTGS) and Its Application to Crop Genetic Improvement
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摘要 转录后基因沉默(PTGS)或RNA干扰(RNAi)技术的发展为创造植物遗传变异体提供了新途径。PTGS于1 998年被明确为双链RNA(dsRNA)诱导的序列特异性基因沉默,短短几年内有关研究取得了突破性进展。结合利用PTGS技术进行的淀粉合成关键酶基因沉默研究,概述了PTGS的作用机理和特点、dsRNA表达载体设计、沉默效应的遗传稳定性及在作物改良应用方面的研究进展。研究表明,基因沉默效应可在子代间稳定遗传并可通过杂交进行重组,显示了其在农作物改良方面的应用潜力。 The dramatic progress in PTGS or RNAi has demonstrated its potential value for creating plant mutants. PTGS was defined as sequence-specific gene silencing triggered by double-strand RNA in 1998. Here, the progress in mechanism of PTGS, the construct design of intron splicing hpRNA for plant transformation and expression, the phenotypic stability of the gene silencing over generations and the application of PTGS to crop genetic improvement were summarized. The gene silencing effects could be inherited stably over several generations and the changed traits could be recombined by crossing, making this approach a reliable technique for genetic modification of crops.
作者 黄冰艳 吉万全 郭蔼光 Sadequr R 李忠宜
机构地区 西北农林科技大学农学院 西北农林科技大学生命学院 Plant Industry
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第5期1-5,共5页 China Biotechnology
基金 国家留学基金管理委员会资助项目 (2 18410 46)
关键词 转录后基因沉默 PTGS 作物 遗传改良 作用机理 RNA干扰 反向遗传学 PTGS RNAi Reverse genetics dsRNA hpRNA Crop genetic improvement
分类号 Q753 [生物学—分子生物学] S33 [农业科学—作物遗传育种]
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参考文献20

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二级参考文献81

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中国生物工程杂志
2005年 第5期

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