半叶马尾藻多糖对辐射损伤小鼠骨髓细胞集落生成的影响

Effect of Sargassum Hemiphyllum Polysaccharides on the Colony-Forming Unit of Bone Marrow Cells in Irradiated Mice

探讨半叶马尾藻多糖[Sargassumhemiphyllum (Turner)C .Ag .polysaccharides ,SHP]对辐射损伤小鼠骨髓细胞集落生成的影响。检测辐射损伤小鼠脾集落生成单位、红细胞集落生成单位、爆裂型红细胞集落生成单位、粒细胞 巨噬细胞集落生成单位、巨核细胞集落生成单位、混合集落生成单位的变化。5Gyγ射线照射后小鼠CFU S ,CFU E ,BFU E和CFU Mix明显减少,而SHP(2 0mg/kg～40mg/kg)可以抑制CFU S ,CFU E ,BFU E和CFU Mix下降。辐射损伤也可以引起小鼠CFU GM显著降低,但是,SHP(10mg/kg～40mg/kg)具有拮抗辐射损伤的作用,使CFU GM增加。单纯照射组小鼠CFU Meg显著低于正常对照组,SHP(4 0mg/kg)加照射组小鼠CFU Meg却显著高于单纯照射组。SHP对辐射损伤小鼠多能干细胞和造血祖细胞具有保护作用。

The object is to investigate the effect of SHP(Sargassum hemiphyllum polysaccharides) on the hematopoietic function in irradiated mice. The methylcellulose culture method was used to detect the CFU-S, CFU-E, BFU-E, CFU-GM, CFU-Meg and CFU-Mix in the irradiated mice. It was found that the numbers of CFU-S, CFU-E, BFU-E and CFU-Mix were significantly reduced after irradiation of 5Gy γ-rays. However, SHP at dosage 20mg/kg～40mg/kg could inhibit their decrease. The development of CFU-GM was also decreased by the irradiation and increased by SHP(10mg/kg～40mg/kg)which demonstrated the radioprotective effect of SHP. The number of CFU-Meg was significantly lowered in the irradiated group than in the control group and higher in the SHP(40mg/kg) plus irradiated group compared with the irradiated group. The conclusion can be drawn that SHP has a protective effect on the cord blood hematopoietic stem cells in irradiated mice.