轮状病毒微量核酸检测技术研究进展

Advances in Detection Technologies of Trace Rotavirus Nucleotide

同经典的免疫学检测方法相比,现代分子生物学技术用于检测轮状病毒具有更高的灵敏性,特异性和样品检测的广泛性。本文主要介绍了聚丙烯酰胺凝胶电泳法,RT-PCR相关的扩增技术和核酸杂交技术,限制性片段长度多态性分析(RFLP),着重讨论了新兴的实时荧光定量PCR,基因芯片技术,核酸序列依赖性扩增(NASBA)技术等。并对各种方法的灵敏性,特异性等进行了比较。

Compared with the classical immunological detection methods, the modern molecular biotechnology has a higher sensitivity, specificity and sample universality for the detection of rotavirus. The article reviews polyacrylamide gel electrophoresis (PAGE), amplification methods based on RT-PCR and nucleotide hybridization technique, restriction fragment length polymorphism assay (RFLP), especially the new methods including real time reverse transcription-polymerase chain reaction, gene chip and nucleic acid sequence-based amplification (NASBA). The review also compares these techniques in terms of their sensitivity and specificity and other features.