摘要
目的:探讨γ射线照射对单采血小板保存过程中血小板活化的影响。方法:用细胞分离机单采血小板20人份,以27Gyγ射线照射,标准条件下保存5天,间隔取样分别用RIA,ELISA方法检测血小板表面P-选择素(P-Selectin)、血浆中可溶性P-选择素(sP-Selectin)及β-血小板球蛋白(β-TG)含量。结果:在保存3d,5d后,照射组及对照组中血小板表面P-Selectin含量、血浆中sP-Selectin及β-TG含量与保存前比较差异显著(P<0.05),但是两组间比较无统计学差异(P>0.05),结论:γ射线照射不会促进血小板的活化。
Purpose: To investigate the platelet activation of apheresis platelets irradiated by gamma ray. Materials and Mothods: Twenty apheresis platelets were prepared by cell separator and irradiated by γ-ray at the dose of 27 Gy, then stored up to 5 days on standard conditions, samples were taken at another day. Contents of P-Selectin on platelet surface,soluble P-Selectin (sP-Selectin) and β-thromboglubulin(β-TG) in plasma were analysed by RIA and ELISA respectively. Results: After storage 3d, 5d, contents of P-Selectin on platelet surface, sP-Selectin and β-TG in plasma of the irradiated and control group have obvious difference compared with that of prestorage(P <0.05),but the comparison between two groups have no difference(P >0.05).Conclusions: γ-ray irradiation have no effect on platelet activation.
出处
《中国医学物理学杂志》
CSCD
2005年第3期537-538,共2页
Chinese Journal of Medical Physics
