HTLV-Ⅰ相关性HAM/TSP患者血浆及脑脊液sFas、sFasL的测定及意义

Serum and CSF Levels of sFas and sFasL in Patients with HTLV-Ⅰ Associated Myclopathy/Tropical Spastic Paraparesis

目的探讨人嗜T淋巴细胞病毒Ⅰ型相关性脊髓病/热带痉挛性瘫痪(humanT-lymphotropicvirustypeⅠassociatedmyelopathy/tropicalspasticparaparesis,HAM/TSP)患者血浆及中可溶性Fas(sFas)和Fas配体(sFasL)表达水平及其与HAM/TSP病发病机制的关系。方法应用ELISA方法检测HAM/TSP患者血浆及脑脊液(CSF)中sFas和sFasL水平。结果HAM/TSP患者血浆sFas表达水平[(1.08±0.26)ng/mL]与其他神经系统疾病(otherinflammatoryneurologicdiseases,OIND)[(0.29±0.05)ng/mL]及正常对照组[(0.18±0.07)ng/mL]相比显著增高(P<0.05),血浆sFasL表达水平[(0.26±0.11)ng/mL]与OIND组[(0.22±0.04)ng/mL]及正常对照组[(0.21±0.05)ng/mL]比较差异无显著性(P>0.05);CSFsFas与sFasL表达水平[(0.19±0.07)ng/mL与(0.24±0.08)ng/mL]均高于OIND组[(0.11±0.06)ng/mL与(0.01±0.01)ng/mL](P<0.05)。结论sFas、sFasL蛋白可能在HAM/TSP发病机制中起重要作用。

Objective To explore soluble Fas (sFas) and Fas Ligand (sFasL) expression in serum and cerebrospinal fluid (CSF) from human T-lymphotropic virus type-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients and their relation to the pathogenesis of HAM/TSP. Methods We evaluated the presence of sFas and sFasL in the sera and CSF of patients with HAM/TSP using an enzyme-linked immunosorbent assay (ELISA). Results Significantly increased serum levels of sFas were found in patients with HAM/TSP than those with other inflammatory neurologic diseases (OIND) and controls (P<0.05). Serum levels of sFasL from patients with HAM/TSP showed increasing trend as compared with patients with OIND and controls (P >0.05). Levels of sFas and sFasL in CSF from patients with HAM/TSP were both significantly increased than those from OIND (P <0.05). Conclusions These results suggest that sFas and sFasL may play a role in the pathogenesis of HAM/TSP.