中国丙型肝炎病毒基因型的进化树分析

The phylogenetic trees analysis of HCV genotypes in China

目的了解丙型肝炎病毒的基因型在中国的分布,对各基因型做遗传进化树分析。方法分型方法按5′端非编码区(5′NCR)ABC程序酶切分型技术进行。应用BsrBⅠ、HaeⅡ、HinfⅠ、BstUⅠ、HaeⅢ等5种限制性内切酶,对HCV阳性血清进行分型研究。对1a、3b基因型进行HCVNS5bPCR,测序。对5′NCR和NS5b进行遗传进化树分析。结果测序结果证实中国存在HCV3b和1a型;进化树分析结果表明第86、98、123株样品与3bD49374相关,获美国NCBI的GenBank认证;第61株与HC J1D107491a型相关;5′NCR测序结果证实第16、62株与HC J82b型相关。结果表明中国存在着HCV1a、2b、3b型的感染。结论经过遗传进化树分析证实了ABC程序酶切分型技术可准确地检测1a～6aHCV基因型。

Objectives To analyze the phylogenetic trees for understanding the distribution of different hepatitis C virus (HCV) genotypes in China.Methods Typing of HCV was performed by a combination of three restriction endonuclease digestion technique (ABC programs) for HCV 5′-NCR HCV.HCV NS5b in the represent isolates of genotype 1a and 3b was amplified and sequenced.Results The sequence analysis indicated genotype 3b and 1a of HCV existed in China,which have have not been reported previously in Chinese patients with HCV infection.The results of phylogenetic analysis for 5′-NCR and NS5b were in agreement with the sequencing reports.Conclusion The ABC program of restriction endonuclease digestion technique for HCV 5′-NCR is accurate and efficient for detection of HCV and discrimination of HCV genotypes 1a-6a.