摘要
利用密度梯度离心等方法从孵化51～56h的石歧杂鸡胚血液中提取PGCs,用自制的玻璃注射针将PGCs注射到孵化2.5d的H系受体鸡胚中制备种系嵌和体鸡;通过筛选AFLP引物建立起家禽嵌和体的AFLP检测方法;经检测20个发育的PGCs受体鸡胚中有8个种系嵌和体,嵌和率为40%。
PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras. AFLP checking method was established to identify chicken germline chimeras. Eight germ-line H-S chimera embryos were identified among 20 developing H breed embryos.
出处
《遗传》
CAS
CSCD
北大核心
2005年第3期367-371,共5页
Hereditas（Beijing)
基金
上海市农业科学院中青年学术技术带头人培养基金资助~~
