流式细胞术分析和分拣植物染色体

Flow Cytometric Analysis and Sorting of Plant Chromosomes

流式细胞术是当染色体、细胞核和细胞等颗粒随着流动的液体(水或缓冲液)通过一个测量点时,被探测器探测到,这样根据颗粒的物理和化学特征而将不同的颗粒分开并计数分拣的技术。流式细胞分析在人类基因组计划中发挥了重要作用,流式细胞技术的应用也适用于植物,目前这个技术应用范围包括流式核型分析,分拣纯化染色体,定位基因,构建文库等。文章综述了流式细胞术在植物基因组分析方面的研究进展。

Flow cytometry refers to the technique where the measurement of the physical and chemical characteristics of particles including chromosomes, nuclei and cells is made as the particles pass in a fluid stream through a measuring point surrounded by an array of detectors. FCM (flow cytometry) analysis has played an important role in human genome projects. This technique is now increasingly used to establish flow karyotypes, sort chromosomes, map genes and construct DNA libraries in plants. This paper reviewed progress in flow cytometric analysis of plant genomes.