阿托伐他汀对内皮细胞内皮脂酶mRNA表达的影响

Effect of atorvastatin on expression of endothelial lipase's mRNA in cultured human umbilical vein endothelial cells

目的:探讨阿托伐他汀对人脐静脉内皮细胞(HUVEC)内皮脂酶(EL)mRNA表达的影响。方法:将阿托伐他汀加入HUVEC培养基,使其终浓度分别为0(对照组)、2、4、6、8、10μmol/L,分别孵育2、4、8、12、24h后,用半定量RT-PCR法检测HUVEC内ELmRNA表达的改变。结果:阿托伐他汀抑制HUVEC内ELmRNA表达。相同时间内,药物浓度越高,ELmRNA表达量越少;相同浓度下,作用时间越长,ELmRNA表达量越少。与对照组相比,差异有统计学意义(P<0.05)。结论:阿托伐他汀抑制HUVEC内ELmRNA的表达,且呈时间-剂量依赖性。

Objective: To evaluate the effect of atorvastatin on mRNA expression of endothelial lipase(EL) in cultured human umbilical vein endothelial cells (HUVEC). Methods: Each group of cultured HUVEC was incubated with atorvastatin of 0, 2, 4, 6, 8 or 10 μmol/L for 2, 4, 8, 12 and 24 hours separately. The expression of ELmRNA in HUVEC was detected by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). Results: Atorvastatin markedly restrained the expression of ELmRNA in HUVEC. During the same period, the expression of ELmRNA was gradually decreased with the increased concentration of atorvastatin. In the same concentration, ELmRNA was reduced with the prolonged action period. Conclusion: Atorvastatin can restrain the expression of ELmRNA, that is dependent on the concentration and time.