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细胞因子对乳腺癌细胞钠碘转运体基因表达的调控 被引量：1

Effect of cytokine on the expression of sodium iodide symporter gene in breast cancer cells
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摘要 目的本实验通过三种细胞因子(肿瘤坏死因子-α,干扰素-γ和白细胞介素-6)对乳腺细胞钠碘转运体(NIS)基因表达的影响,探讨乳腺肿瘤组织NIS的表达特点和调控因素。方法采用乳腺癌细胞MCF-7和MB453进行培养,分别给予不同浓度的肿瘤坏死因子-α,干扰素-γ或白细胞介素-6刺激72h。采用RT-PCR方法检测细胞中NISmRNA表达情况。结果乳腺癌细胞在经过肿瘤坏死因子-α,干扰素-γ或白细胞介素-6刺激后,细胞中NIS的mRNA表达比对照组明显降低,并且与细胞因子浓度呈负相关。结论肿瘤坏死因子-α,干扰素-γ和白细胞介素-6对乳腺癌细胞MCF-7和MB453的NIS基因mRNA表达有抑制作用,这提示细胞因子可能通过调节乳腺癌NIS基因mRNA的表达,从而影响乳腺癌细胞对放射性碘治疗的敏感性。 Objective To investigate the effect of cytokines (TNF-α,IFN-γ and IL-6) on the expression of sodium-iodide symport-er(NIS) gene in breast cancer cells MCF-7 and MB453. Methods The breast cancer cells were cultured with negative control culture and culture with different concentration of cytokine for 72 hours separately. NIS gene mRNAs in breast cancer cells cultured were determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Results Expression of sodium-iodide symporter mRNAs were found decreasing along with increasing the concentration of cytokine dose-dependently. Conclution Cytokine may play a role in iodide-uptake modulating in breast cancer cells by their effect on NIS gene expression.
作者 贾悦 刘超 覃又文 刘翠萍 唐伟 李倩 袁庆欣 茅晓东 狄福松
机构地区 南京医科大学第一附属医院内分泌科
出处 《肿瘤》 CAS CSCD 北大核心 2005年第3期214-216,220,共4页 Tumor
基金 江苏省卫生厅135工程重点学科基金资助项目(编号:苏卫科教2001-31)
关键词 钠碘转动体(NIS) 乳腺肿瘤 肿瘤坏死因子 干扰素-Γ 白细胞介素-6 MCF-7细胞 MB453细胞 Sodium-iodide symporter Breast neoplasms Tumor necrosis lactor Interteron-gamma Interleukin-6 MCF-7 cells MB453 cells
分类号 R737.9 [医药卫生—肿瘤] R73-362 [医药卫生—肿瘤]
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参考文献10

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二级参考文献38

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共引文献10

同被引文献10

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二级引证文献3

肿瘤
2005年 第3期

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