摘要
以甜菜叶柄为外植体,通过农杆菌介导转化,将玉米胚乳核糖体失活蛋白基因(cRIP)导入甜菜,对影响转化频率的因素:卡那霉素的浓度、头孢霉素的浓度、农杆菌感染时间和浓度等进行了探讨,初步建立了转化频率较高的转化系统,当卡那霉素浓度为100mg/L,头孢霉素浓度为500mg/L,农杆菌浓度OD值为0.3～0.4,感染时间为8～10min时,抗性不定芽分化率可达36.5%。该转化系统为转基因植株的获得奠定了基础。
cRIP genes were transfered into sugarbeet via Agrobacterium using petioles as explants. The factors that may affect transformation frequency were investigated, such as concentration of Kanamycin(Kan) and Cefazolin sodium(Cef), infection time and infection concentration of Agrobacterium. When infection concentration was OD=0.3-0.4 and infection time was 8-10 minutes while 100mg/L Kan and 500mg/L Cef were added into the selective medium, high transformation frequency can be obtained. A stable and efficient Agrobacterium mediated transformation system has been established.
出处
《中国糖料》
2005年第1期6-8,共3页
Sugar Crops of China
基金
国家自然科学基金资助项目(No.30060048)
关键词
甜菜
遗传转化
频率
Surgarbeet
Genetic transformation
Frequency
