摘要
在弱碱条件下,磷酸酚二钠会被碱性磷酸酶(ALP)催化水解生成苯酚,苯酚在硫酸铜的催化下能同显色剂2,6—二氯醌氯亚胺(CQC)或2,6--二溴醌氯亚胺(BQC)反应生成稳定的蓝色靛酚,测定兰色靛酚定吸光度,并与标准系列比较定量,就可测定各类乳品中ALP活度,这两种方法可用于区别生鲜乳品和巴氏杀菌乳品,并可有效判定乳制品巴氏杀菌的效果。本文比较了BQC法与CQC法测定乳品的ALP活度,结果显示选择2,6—二氯醌氯亚胺(CQC)作显色剂较为理想。
Detecting ALP activity in dairy products is based on the principle that at weak base condition, disodium phenol phosphate can hydrolyze phenol by activator [Alkaline phosphatase (ALP)]. The phenol can react with 2,6-dichloroquinone chloroimide(CQC) or 2,6-dibromaquinone chloroimide(BQC) to produce indophenol blue catalyzed by copper sulfate. Quantitatively detecting indophenol can calculate ALP activity. It can judge the effect of pasteurization in dairy products and stabilization of organic phosphate compounds in raw milk. This paper compares the CQC method and BQC method. The result showed CQC method is better than BQC method for detecting the ALP activity.
出处
《现代食品科技》
EI
CAS
2005年第2期161-162,160,共3页
Modern Food Science and Technology
