伪狂犬病病毒gE/gBPCR鉴别方法的建立及其应用

Development of PCR for identifying pseudorabies virus gE/gB strains and its application

为了检测伪狂犬病病毒(PRV)潜伏感染及确定病毒潜伏的主要部位,建立了能鉴别PRV野毒株和gE基因缺失疫苗株gE/gB的PCR诊断方法。结果表明,所建立的PCR 特异性强、敏感性高、稳定性好,能用于病毒潜伏感染的检测;同时还确定PRV潜伏感染的主要部位是三叉神经节、扁桃体、嗅球、脑干、脑桥和咽黏膜。

The study was undertaken to develope the gE/gB differential PCR, which could differen- tiate the pseudorabies virus virulent strain from gE gene-deleted vaccine strain and detect the latently infected site. The results showed that the developed gE/gB differential PCR had high sensitivity and specifi- city and could be used to detect the latent infection of pseudorabies virus. Meanwhile, the regular sites where pseudorabies virus virulent strains established latency were determined to be trigeminal ganglion, tonsil, olfactory bulbs, brain stem, thalamus and pharyngeal mucosa.