摘要
从河南省某鸡场中疑似鸡传染性支气管炎(IB)的雏鸡病料中分离到1 株鸡传染性支气管炎病毒(IBV),暂命名为HN,鉴定为IBV。从接种HN毒株的鸡胚尿囊液中提取单股RNA后应用反转录聚合酶链反应(RT PCR)技术扩增得到包含IBV HN株5a、5b蛋白及N蛋白基因的约1 600 bp片段;将克隆测序的5a、5b及N基因分别与GenBank中11 株国内外参考毒株相应基因进行序列比较与遗传变异分析,发现IBV HN株变异独特,明显不同于国内外参考毒株。
The infectious bronchitis virus (IBV) HN strain was successfully isolated from a chicken flock with typical clinical symptom of infectious bronchitis in Henan Province of China. The strain was passaged in SPF chicken embryos, characterized by haemagglutination test and observed by electron microscope. The cDNA fragment including nucleocapsid protein gene, 5a and 5b protein genes of the strain, which is about 1600bp in length, were amplified according to designed specific primers by RT-PCR from viral RNAs. Compared the 5a, 5b genes and N gene respectively with that of the other 11 IBV strains from GenBank on genetic variation, homology of nucleotide and deduced amino acid sequences the IBV HN strain differed from the other strains from GenBank obviously.
出处
《中国兽医科技》
CSCD
北大核心
2005年第5期357-362,共6页
Chinese Journal of Veterinary Science and Technology
基金
黑龙江省教育厅科学技术研究项目( SARS 专项课题)
黑龙江省政府博士后科研启动金资助项目(LRBKY01045)
