氟哌啶醇对小鼠卵母细胞染色体的影响

Effects of Haloperidol on Mouse Oocyte Chromosomes

背景与目的:探讨抗精神病药氟哌啶醇(Haloperidol,Hal)在卵子发生过程中对卵母细胞染色体的影响。材料与方法:将40个12周龄健康雌性小鼠随机分为4组,分别按0、0.05、0.50、5.00mg/(kg·d)腹腔注射Hal,连续注射8d,通过超排收集卵母细胞,制备染色体标本进行核型分析。结果: 对照组、0.05、0.50、5.00mg/(kg·d)组的实验参数分别为,①超单倍体率:3.8 %、4.4 %、7.3 %、18 %;②非整倍体率:7.5 %、8.8 %、14.5 %、36.0 %;③退化卵母细胞率:1.3 %、1.4 %、1.8 %、10.0 %;④染色体分离均数:0.013、0.014、0.018、0.100。0.05、0.50mg/(kg·d)组分别与对照组比较,各实验参数间的差异没有统计学意义(P>0.05)。5.00mg/(kg·d)组各实验参数值均比对照组高,其差异具有统计学意义(P<0.05)。结论:大剂量Hal有可能诱发卵母细胞非整性增加并引起卵母细胞退化。

BACKGROUND&AIM: To investigate the effects of haloperidol(Hal)on oocyte chromosomes in oogenesis. MATERIAL AND METHODS: Forty healthy female mice,12weeks-old,were randomly divided into four groups.The animals of different groups were exposed to Hal by intra-peritoneal injection of0,0.05,0.50and5.00mg/(kg·d)of Hal for successive8days,respectively.Oocytes were collected by superovulation and the metaphase were prepared for karyotypic analysis. RESULTS: For0,0.05,0.50and5.00mg/(kg·d)Hal-groups,the frequencies of hyperhaploid complements were3.8%,4.4%,7.3%,18%,the frequencies of aneuploid complements were7.5%,8.8%,14.5%,36.0%,the frequencies of degenerative oocytes were1.3%,1.4%,1.8%,10.0%and the mean number of splitting chromosomes were0.013,0.014,0.018,0.100,respectively.Comparing all the experimental parameters of the0.05and0.50mg/(kg·d)Hal-groups with those of the control,no significant differences were observed(P>0.05).All the experimental parameters of5.00mg/(kg·d)Hal-group were higher than those of the control,and the differences of the parameters between two groups were statistically significant. CONCLUSION: A large-dose of Hal might be able to induce aneuploidy and degeneration of oocytes in oogenesis.