阿米福汀在肺癌放射治疗时对机体正常细胞和肺癌细胞的影响

The radioprotective effects of amifostine on normal tissue cells and Lewis lung cancer

目的 探讨阿米福汀对体外培养的中国仓鼠V79细胞和C5 7BL/ 6小鼠造血系统的放射保护作用及其在肺癌放射治疗时对肺癌组织生长的影响。方法 将体外培养的中国仓鼠V79细胞接种于培养皿后,分别给予2～10Gy的γ射线照射,用药组在照射前30min暴露于阿米福汀(1 2mmol/L) ,评价阿米福汀对照射后V79细胞存活分数的影响;给予C5 7BL/ 6无瘤小鼠腹腔注射阿米福汀(2 0 0mg/kg) 30min后进行全身照射(8Gy) ,在照射后不同时间观察外周血白细胞数量的变化;给予荷Lewis肺癌C5 7BL/ 6小鼠肿瘤局部照射10Gy ,照射前30min腹腔注射阿米福汀(2 0 0mg/kg) ,评价阿米福汀对肿瘤放疗效果的影响。结果 用药组V79细胞的集落存活分数高于单纯放射组(P <0 0 5 ) ;用药组无瘤小鼠外周血白细胞数较单纯放射组下降幅度小且恢复快(P <0 0 5 ) ;在观察的2 0d内,比较用药组和单纯放射组荷Lewis肺癌小鼠肿瘤体积,在相同时间点两组之间比较无显著性差异(P >0 0 5 )。结论 本研究从离体和在体两方面证实了阿米福汀对机体正常细胞产生放射保护的同时。

Objective To evaluate the radioprotective effects of amifostine against radiation-induced injury on cultured V79 cells in vitro and haematopoietic progenitor cells and Lewis lung cancer tissue in vivo.Methods The cultured Chinese hamster V79 cells were irradiated with 2～10 Gy of γ rays after exposure to 1.2 mmol/L of amifostine for 30 min and the survival fractions were evaluated.Amifostine (200 mg/kg) was administered intraperitoneally to C57BL/6 mice before the whole body irradiation with 8 Gy of γ rays and the white blood cell counts were measured.C57BL/6 mice bearing Lewis lung cancer cells were locally irradiated with a single dose of 10 Gy for 30 minutes after amifostine (200 mg/kg) administration intraperitonially.Results A brief exposure to amifostine protected cultured V79 cells from radiation injury.Amifostine also conducted radioprotection to haematopoietic progenitor cells of normal C57BL/6 mice.However,no reduced radiation injury effect on Lewis lung cancer tissues was observed.Conclusion Amifostine protects normal cells from radiation damage wihtout extenuation of tumoricidal potential against Lewis lung cancer.