荧光定量PCR方法探讨黄芪注射液对巨噬细胞吞噬结核杆菌的影响

Effect of Astragalus root injection on macrophagal phagocytosis of mycobacterium tuberculosis

目的: 探讨黄芪注射液对巨噬细胞吞噬结核杆菌的作用.方法: 以浓度为0 (对照组), 0 .05, 0. 2, 0. 6, 1. 5,4g/L的黄芪注射液100μL与RPMI1640灌洗小鼠腹腔获得的巨噬细胞及结核杆菌共同孵育,采用荧光定量PCR法检测巨噬细胞对结核杆菌的吞噬能力.结果: 在黄芪注射液浓度为0. 2, 0 .6, 1. 5, 4g/L时,黄芪对巨噬细胞吞噬结核杆菌有明显的促进作用.并且以1. 5g/L浓度为最佳.结论: 黄芪可以提高巨噬细胞对结核杆菌的吞噬作用,并具有双相性,当其浓度过高时具有抑制巨噬细胞吞噬结核杆菌的作用.本研究为临床治疗结核病提供了理论依据和新的思路.

AIM: To explore the mechanism by which Astragalus root injection affects the macrophagal phagocytosis of mycobacterium tuberculosis.METHODS: Macrophages and mycobacterium tuberculosis were extracted from mice by washing mouse peritoneum with RPMI1640.Astragalus root injection at different concentrations (0,0.05,0.2,0.6,1.5 and 4 g/L) was coincubated.The capacity of macrophagal phagocytosis of mycobacterium tuberculosis was detected by FQ-PCR method.RESULTS: At the concentration of 0.2,0.6,1.5 and 4 g/L,Astragalus root injection enhanced the capacity of macrophagal phagocytosis of mycobacterium tuberculosis.At the concentration of 1.5 g/L,the effect was the most significant.CONCLUSION: Astragalus can enhance the capacity of macrophagal phagocytosis of mycobacterium tuberculosis and this mechanism is bi-directional.Astragalus can inhibit macrophagal phagocytosis of mycobacterium tuberculosis at too high a concentration.This research provides some new insight into the clinical treatment of tuberculosis.