摘要
目的: 研究益气活血化瘀中药复方制剂芪丹通脉片(qidantongmaitablet,QDTMT)对血管内皮细胞(vasculaen dothelialcells,VEC)抗血栓形成作用的影响,进一步探讨益气活血化瘀药物的作用机制.方法: 48只SD大鼠随机分为空白对照组(Control)、模型组(Model)、QDTMT高剂量组(QDT -MTH)、QDTMT中剂量组(QDT-MTM)、QDTMT低剂量组(QDT MTL)和阳性对照组(Herbesser),用QDTMT(或生理盐水)给SD大鼠连续ig14d,第15日起注射大剂量肾上腺素,复制血管内皮细胞损伤模型,全自动血液凝固法检测抗凝血酶-Ⅲ(AT- Ⅲ)和血管性血友病因子(vWF)的活性,放免法检测6-酮-前列环素-F1α(6- Keto- PGF1α)和血栓素B2 (TXB2 )的含量.结果: 益气活血化瘀中药复方制剂QDTMT能显著降低血管内皮细胞损伤大鼠血浆vWF活性和TXB2 的水平,显著升高AT Ⅲ活性, 6- Keto -PGF1α水平和6- Keto -PGF1α/TXB2 比值,与模型组相比有显著差异(P<0 .01),且QDTMTH明显优于QDTMTM和Herbesser.结论: QDTMT对内皮细胞损伤具有保护作用,增强血管内皮细胞抗血栓功能和减低止血功能,这可能是QDTMT益气活血化瘀的作用机制之一.
AIM: To study the effects of QiDanTongMai tablet (QDTMT),a compound preparation made up of pure traditional Chinese herbs,on the anti-thrombosis function of endothelial cells in rats with vascular endothelial injury,and to further explore the mechanism of reinforcing qi and activating blood circulation to remove blood stasis.METHODS: Forty-eight SD rats were divided randomly into Control,Model,QDTMT high dosage (QDTMT_H) and moderate dosage(QDTMT_M),low dosage(QDTMT_L)and herbesser (Herbesser) groups.The suspension of QDTMT was given to SD rats by gastrotube for 2 weeks and the model of vascular endothelial was established by injecting subcutaneously a high dose of adrenaline solution to the SD rats on the 15th day.The activity of AT-Ⅲ and vWF and the concentration of 6-Keto-PGF1a and TXB_2 were determined by automatic blood coagulation and radioimmunoassay respectively.RESULTS: Compared to those of the model group,the activity of AT-Ⅲ and the concentration of 6-Keto-PGF1a and the ratio of 6-Keto-PGF1a/TXB_2 increased.The activity of vWF and the concentration of TXB_2 were lowered significantly in the group of QDTMT_H and QDTMT_M and the effects of QDTMT_H were obviously better than those of QDTMT_M and Herbesser.CONCLUSION: QDTMT can prevent vascular endothelium from injury, increase the anti-thrombosis function and decrease the hemostasis function of endothelial cells,which may in part be responsible for QDTMT in reinforcing qi and activating blood circulation to remove blood stasis.
出处
《第四军医大学学报》
北大核心
2005年第10期948-950,共3页
Journal of the Fourth Military Medical University
关键词
芪丹通脉片
内皮
血栓
QiDanTongMai tablet
endothelial
thrombosis