健乳灵治疗大鼠乳腺增生的病理观察

Pathological Evaluation of Mammary Gland Hyperplasia Rats Treated with Jianruling

目的:研究健乳灵对乳腺增生大鼠的治疗作用及对乳腺组织p16、p53表达的影响。方法: 75只大鼠随机分为5组,分别为正常对照组,模型组,高、中、低三个药物试验组。分别用不同剂量的健乳灵治疗60d,取乳腺组织观察乳腺的病理变化和p16、p53蛋白表达。结果:健乳灵三个剂量组均能抑制乳腺上皮增生,减少小叶和腺泡数目,降低大鼠乳腺增生组织的p16、p53蛋白表达水平, 与模型组比较有显著性差异(P<0. 01)。结论:健乳灵对大鼠乳腺增生病有调节和治疗作用。

Objective:To study the effect of Jianruling on the expression of p16 and p53 in rats with mammary gland hyperplasia. Methods:The model of mammary gland hyperplasia in female rats was established by intramuscular injection of estradiol and progesterone. The pathological changes of mammary gland were observed under light microscope,and the level of expression of p16 and p53 were measured by immunohistochemical method after treatment with Jianruling at dose of 0.842 g/(kg·d),1.684g/(kg·d) and 3.368 g/(kg·d) for 60 days. Results:Jianruling can inhibit mammary gland epithelial hyperplasia, reduce the quantity of lobules and acini,while the level of expression of p16 and p53 with mammary gland hyperplasia significantly decreased to lower than that of the model control group(P<0.01). Conclusion:Jianruling has positive effect on the adjustment and treatment of rats with mammary gland hyperplasia.