采用差异显示法分离支气管哮喘病因多基因初探

Differential Display of Asthma-Related Genes by Reverse-transcriptase PCR

目的:初步分离出在哮喘动物模型中发生差异显示的基因条带,为对哮喘的进一步研究打下基础。方法:腹腔注射卵清蛋白复制哮喘动物模型,采用差异显示反转录PCR(DDRT PCR)方法初步分离出发生差异显示的基因条带。结果:哮喘模型组豚鼠耗氧率明显高于对照组,具有明显差异的条带有30条。结论:这些条带所起作用尚需进一步的分析验证。

Objective:To probe the asthma-related genes by differential display reverse-transcriptase PCR(DD RT-PCR). Methods :Set up animal model of asthma by OVA injection, then genes were displayed by DD RT-PCR. Results: About 30 fragments were different.Conclusion:The function of these fragments needs further research.