海水中铜在扇贝组织的蓄积及其对酶活性的影响

Accumulation of copper in the tissues of Chlamys farreri and its effects on the activity of superoxide dismutase in the seawater

采用暴露实验方法,研究了不同浓度的铜(Cu2+)对栉孔扇贝(Chlamysfarreri)鳃、肌肉、内脏团组织铜蓄积量及超氧化物歧化酶(SOD)活性的影响。结果表明:随水体中铜浓度升高,扇贝各组织的铜蓄积量明显上升,依次为内脏>鳃>肌肉。Cu2+浓度对栉孔扇贝各组织超氧化物歧化酶(SOD)活性均有明显影响,酶活性随Cu2+浓度升高表现为抑制-诱导-抑制的规律。栉孔扇贝各组织SOD酶对水体中Cu污染反应敏感,存在计量-效应关系,对海洋铜污染具有指示作用。能灵敏指示海洋铜污染的扇贝优选组织是鳃,其次是内脏。

An exposure experiment was conducted to study the effects of different concentrations of copper (Cu2+) on copper accumulation and activities of Super Oxide Dismutase (SOD) in gills, muscle and viscera of Chlamys farreri. The results showed that copper accumulation in the tissues increased significantly with the increase of copper concentration in the seawater, the order of copper accumulation in the tissues of Chlamys farreri was as follows from high to low: viscera, gills and muscle. Copper concentration has significant effect on the activity of SOD and it indicates the law of restraint-induction-restraint with the increase of copper concentration. Such results show that the activity of SOD responses sensitively to the copper contamination and it is a reliable indicator of copper pollution. The optimum selective tissue for the indication of copper contamination on Chlamys farreri should be gills, followed by viscera.