利用RAPD和同工酶研究中国兜兰属种间亲缘关系

The Relationship of Paphiopedilum Species in China by RAPD and Isozyme

本文选择14种10个碱基的寡核苷酸引物和7种酶系统,应用RAPD和同工酶分子标记技术分析中国兜兰属(Paphiopedilum)种间亲缘关系,并与陈心启和Cribb的传统分类、Cox等的ITS结果进行比较。研究结果显示,RAPD和同工酶两种手段对种间亲缘关系的划分与传统分类大致吻合,同工酶比RAPD的结果更接近传统分类。兜兰亚属需进一步分组,彩云兜兰、卷萼兜兰、紫纹兜兰及其变种云南虎皮应自成一个组。

RAPD and isozyme analyses by 14 primers,which are 10 oligonucleotides,and 7 isozyme systems were used to the study of the relationship among species of Paphiopedilum in China. Meanwhile the classifications of Paphiopedilum got from traditional classification(Chen's and Cribb's), ITS(Cox's), RAPD and isozyme(Our's)are compared in this paper. The result shows that the relationship of the subgenus revealebs isozyme dater is more similar to traditional classification than that of RAPD dater. Our results exhibited that the Subgenus Paphiopedilum need to be subdivided into several sections.The species P.wardii, P. appletonianum, P. purpuratum, P. ‘Yunnahupi’ are wallcuted its own section.