应用聚合酶链反应检测乙肝患者肾组织中的HBV DNA

DETCTION OF HBV DNA IN KIDNEY BY PCR

应用套式聚合酶链反应(PCR)技术对11例乙肝患者石蜡包埋的肾、肝组织中的HBV DNA进行了检测,并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明:肾组织中HBV DNA的检出率为8/11(72.7％),8例中包括膜性肾炎和膜增生性肾炎各1例,均不存在有HBV的复制;在肝组织中均有HBV感染,其中5例有HBV复制。HBV可以感染肾组织但并不在肾组织中复制这一结果支持乙肝患者肾脏出现的病理改变是由于沉积于肾小球内的免疫复合物介导损伤的结果。

Kidney and. liver tissues fixed with formalin and psraffinembedded in eleven autopsy patients with HBV infection were studiedby nested polymmerase chain reaction (PCR) for detection of HBV DNA with two groups of primer. The DNA sequence representing HBV infectionwas detected in all liver tissues (100%) and in eight kidney tissues (72. 7%), respectively. The DNA sequence represented HBV replicationwas detected in 5 cases of liver tissues only. The findings of PCR were compared with the results of immunohistochemistry and hybridization in situ. The result revealed that HBV could infect kidney but does not replicate in it. It suggests that histopathologicalchange of glomerulonephritis in the patient with HBV infection maydeposited in glomeruli.