异型增生在小鼠大肠腺癌发生中的研究

The Dysplasia in Carcinogenesis of the Adenocarcinoma of the Colon in Mice

二甲肼给昆明种雌性成年小鼠每周皮下注射1次（20mg／kg），最多20次。在小鼠大肠腺癌诱发过程中．用常规组织学和酶组织化学技术对异型增生腺体进行了动态观察，发现异型增生腺体于实验第3周出现，以后逐步增多。异型增生腺体病变可累及单个腺体的部份区域、单个腺体或邻近的多个腺体，以后发展为突向肠腔的灶性病变。其酶变化表现为LDH、G6PD、β-Gr活性增高，CCO、SDH活性下降。文中对异型增生腺体在小鼠大肠腺癌发生中的地位进行了讨论。

The Kunming female adult mice were given subcutaneous injection of DMH (20mg/kg) weeklyand killed at various intervals after 7 days of the injection. The histopathology and the enzyme activityof the dysplasia were investigated by means of hematoxylin and eosin stain and histochemistry. By the3rd week after initation of stain DMH treatment, the isolated dysplastic crypt in one mouse was ob-served and the numbers of the dysplastic crypts had increased in subsequent mice. The dysplasia in-volved the partial area of single crypt or single crypt or several crypts in the vicity. The dysplasia showed tortuosity, budding, and branching in the cryptal cavity and formed foci (cup-like, round or triangular shap) in the mucosa. The latter dysplasia expanded polypiform or nodular foci protrusively in the colon cavity. The enzyme activity of the dysplasia showed LDH, G6PD, β-Gr increased and CCO, SDH decreased, The nature and meaning of the dysplasia were discussed in this paper.