固定化酶在高浓度有机废水处理中的应用

Application of Immobilized Proteases in Treating Water with High Concentration Organic Substance

选用硅胶、活性炭、大孔树脂三种载体,在一定条件下用物理吸附法固定蛋白酶,三种载体固定的蛋白酶对含高浓度蛋白质的淀粉黄浆废水进行水解实验,确定水解最佳的pH值范围,并在此条件下研究三种不同载体上固定化酶的活性及其水解规律。结果表明:大孔树脂对蛋白酶的固定效果良好,并对含高浓度蛋白质的废水处理效果最好,其最佳反应pH值为5.5,反应进行0.5 h时酶的催化效果最好,氨基酸产生速度达到30.82 mg/min,且在3h后该速度仅减少3.74 mg/min;6 h时对蛋白质的去除率达到39.93%。

Under a certain conditions, the experiment immobilizes proteases on silica gel, activated charcoal and large-pore resin by physical adsorption, and then compares the immobilizing effect of the three supports to find out the optimal support. Subsequently, proteases on three different supports are used in enzymatic hydrolysis of soybean wastewater with highly-concentrated protein in order to investigate the optimal pH for enzyme activity and treatment effect. At last, on optimal pH condition, study on the evolution of enzyme activity and treatment effect with the alteration of hydraulic retention time (HRT) is conducted. Comparing the immobilizing effect, enzyme activity and treatment effect at different pH and the evolution of enzyme activity and treatment effect with the alteration of HRT on the three supports, it can be concluded that resin has good immobilizing effect and best treatment effect on wastewater with highly-concentrated protein. The optimal pH is 5.5, enzyme activity reaches the peak 30.82 mg/min at 0.5 h, and the loss of enzyme activity is relatively small, only 3.74 mg/min. The protein reduction ration is 39.93%.