脂多糖和细胞因子刺激肾小球系膜细胞合成PAF的研究

Cytokine- and LPS-induced syntbesis ofplatelet activating factor from glomerularmesangial cells

本研究首先建立了血小板活化因子（ＰＡＦ）生物活性检测法，并测定了体外培养的系膜细胞经ＩＬ－１β，ＩＬ－６和ＴＮＦα刺激后，其培养上清中ＰＡＦ的含量。结果发现，上述细胞因子均能刺激系膜细胞合成ＰＡＦ，并呈一定的剂量依赖关系，ＩＬ－１β，ＩＬ－６和ＴＮＦα通过这一途径而间接地发挥病理作用，加剧肾小球损害。

n this study, the 3rd parsaged mesan-gial cells (MCs) from rat glomeruli wereseeded in a 24-well plate (5 ×105 cell/well).Then , the indicated concentration of LPS,IL-lβ, IL-6 and TNFα were added. After0. 5 h , the supernatants were collected forPAF assay. PAF was quantitated after ex-tractron and purification by thin layer chro-matography according to the aggregation ofwashed rabbit platelets as reported. The re-sult suggested that LPS, IL-lβ, IL-6 andTNFα stimulate the syhthesis of PAF byMCs in a dose-dependent manner.