血红素氧合酶1在变应性鼻炎豚鼠模型的鼻黏膜中的表达

Expression of heme oxygenase-1 in nasal mucosa of allergic rhinitis guinea pig

目的:通过制备豚鼠变应性鼻炎动物模型,观察并分析内源性一氧化碳(CO)的限速酶血红素氧合酶1(HO1)在豚鼠鼻黏膜组织中的表达。方法:分别以卵清蛋白致敏制备豚鼠变应性鼻炎动物模型为致敏组及以地塞米松处理作为治疗组,以生理盐水处理作为正常对照组,取豚鼠鼻黏膜,苏木精伊红染色观察炎性细胞浸润,免疫组织化学染色方法观察HO1在各组豚鼠的鼻黏膜组织中的表达情况。结果:在黏膜组织中,炎性细胞的浸润与炎症程度有关,炎性细胞以嗜酸粒细胞(EOS)浸润明显,HO1主要表达在黏膜腺上皮细胞的胞质中,3组中,致敏组鼻黏膜HO1表达明显增强(P<0.01),黏膜下EOS浸润明显(P<0.01),激素治疗组HO1的表达弱于致敏组(P<0.01),黏膜下EOS浸润程度减轻,但强于对照组(P<0.01),组间差异有统计学意义(均P<0.01)。结论:HO1主要表达于鼻黏膜的腺上皮细胞质内,豚鼠变应性鼻炎模型中HO1的表达与炎症的严重程度相关,提示内源性CO可能参与了变应性鼻炎的炎症过程。

Objective:To study the expression of a specific inhibitor of endogenous carbon monoxide(CO) generating enzyme Heme oxygenase -1 (HO-1 ) in allergic rhinitis guinea pigs.Method:Guinea pigs were divided into three groups,one were sensitized by using ovalbumin intraperitoneal and nasal challenge,the second group were treated with dexamethasone as therapy group,the third group were treated with saline for control.The infiltration of eosinophil (EOS) with HE staining and the expression of HO-1 by immunohistochemical staining were observed in nasal mucosa.Result:Most of infiltrating cell in mucosa were EOS and the amount of infiltrated EOS related to the level of inflammation,and HO-1 was found mainly located in cell plasma off glandular epithelium of mucosa.The sensitized groups showed that the highest optical densities of HO-1 (P<0.01) and the most EOS(P< 0.01 ) were in epithelium,and the therapy group showed lower optical densities of HO-1(P<0.01) and few of EOS (P<0.01) than sensitized group,but higher than the control group.There was a significant difference between the three groups(P<0.01).Conclusion:HO-1 is mainly located in cell plasma of glandular epithelium of nasal mucosa, and the degrees of expression of HO-1 positive correlate with the process of inflammation in allergic rhinitis guinea pigs,which suggests that the endogenous carbon monoxide might play a significant role in the pathogenesis of allergic rhinitis.